产品详情

  • 产品名称：Mouse HMGCR (3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) Sandwich ELISA Kit Ready-To-Use (STJE0015860)

  • 产品型号：STJE0015860
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse HMGCR (3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) Sandwich ELISA Kit Ready-To-Use (STJE0015860)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.31ng/mL
Detection Limit: 0.781-50ng/mL
Short Description: This HMGCR Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Hmgcr
Gene ID: 15357
Uniprot ID: HMDH_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2. The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. Phosphorylated. Phosphorylation at Ser-871 reduces the catalytic activity.
Function Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalsonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis.
Protein Name 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase
Hmg-Coa Reductase
Database Links Reactome: R-MMU-191273
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Peroxisome Membrane
Alternative ELISA Names 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase ELISA kit
Hmg-Coa Reductase ELISA kit
Hmgcr ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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