产品详情

  • 产品名称：Mouse EGR1 (Early Growth Response Protein 1) CLIA Kit (STJC0001198)

  • 产品型号：STJC0001198
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse EGR1 (Early Growth Response Protein 1) CLIA Kit (STJC0001198)
详情介绍：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This mouse EGR1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Egr1
Gene ID: 13653
Uniprot ID: EGR1_MOUSE
Specificity: This kit recognizes Mouse EGR1 in samples. No significant cross-reactivity or interference between Mouse EGR1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in lung vasculature and in mononuclear phagocytes. Detected in liver (at protein level). Detected in lung vasculature and in mononuclear phagocytes. Expressed in the liver in a circadian manner.
Function Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia.regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosesynthesis of luteinizing hormone (LHB) in the pituitary. Regulates the amplitude of the expression rhythms of clock genes: BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene BMAL1 in the suprachiasmatic nucleus (SCN).
Protein Name Early Growth Response Protein 1
Egr-1
Nerve Growth Factor-Induced Protein A
Ngfi-A
Transcription Factor Zif268
Zinc Finger Protein Krox-24
Cellular Localisation Nucleus
Cytoplasm
Alternative CLIA Names Early Growth Response Protein 1 CLIA kit
Egr-1 CLIA kit
Nerve Growth Factor-Induced Protein A CLIA kit
Ngfi-A CLIA kit
Transcription Factor Zif268 CLIA kit
Zinc Finger Protein Krox-24 CLIA kit
Egr1 CLIA kit
Egr-1 CLIA kit
Krox-24 CLIA kit
Specificity This kit recognizes Mouse EGR1 in samples. No significant cross-reactivity or interference between Mouse EGR1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-113 106
EDTA plasma(n=8) 95-108 100
Cell culture media(n=8) 99-114 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 101.83 301.66 744.82 98.54 314.20 686.16
Standard deviation 11.67 29.20 48.93 12.44 31.48 67.04
CV (%) 11.46 9.68 6.57 12.62 10.02 9.77
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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