产品详情

  • 产品名称：Mouse CTSK (Cathepsin K) CLIA Kit (STJC0001137)

  • 产品型号：STJC0001137
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse CTSK (Cathepsin K) CLIA Kit (STJC0001137)
详情介绍：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This mouse CTSK kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Ctsk
Gene ID: 13038
Uniprot ID: CATK_MOUSE
Specificity: This kit recognizes Mouse CTSK in samples. No significant cross-reactivity or interference between Mouse CTSK and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Predominantly expressed in bones. Expressed in thyroid epithelial cells.
Function Thiol protease involved in osteoclastic bone resorption. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
Protein Name Cathepsin K
Database Links Reactome: R-MMU-1442490
Reactome: -MMU-1474228
Reactome: -MMU-1592389
Reactome: -MMU-1679131
Reactome: -MMU-2132295
Reactome: -MMU-8939242
Cellular Localisation Lysosome
Secreted
Apical Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative CLIA Names Cathepsin K CLIA kit
Ctsk CLIA kit
Specificity This kit recognizes Mouse CTSK in samples. No significant cross-reactivity or interference between Mouse CTSK and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 95
EDTA plasma(n=8) 84-96 91
Cell culture media(n=8) 91-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.58 65.17 199.95 25.56 68.21 212.97
Standard deviation 2.27 6.35 14.58 3.03 5.14 20.36
CV (%) 8.87 9.74 7.29 11.85 7.54 9.56
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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