产品详情

  • 产品名称：Mouse Il1b (Interleukin-1 beta) Sandwich ELISA (STJE0004230)

  • 产品型号：STJE0004230
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse Il1b (Interleukin-1 beta) Sandwich ELISA (STJE0004230)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This Il1b Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Il1b
Gene ID: 16176
Uniprot ID: IL1B_MOUSE
Specificity: This kit recognizes Mouse IL-1 Beta in samples. No significant cross-reactivity or interference between Mouse IL-1 Beta and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Protein Name Interleukin-1 Beta
Il-1 Beta
Database Links Reactome: R-MMU-448706
Reactome: -MMU-5620971
Reactome: -MMU-5660668
Reactome: -MMU-9020702
Cellular Localisation Cytoplasm
Cytosol
Secreted
Lysosome
Extracellular Exosome
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative ELISA Names Interleukin-1 Beta ELISA kit
Il-1 Beta ELISA kit
Il1b ELISA kit
Specificity This kit recognizes Mouse IL-1β in samples. No significant cross-reactivity or interference between Mouse IL-1β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 96
EDTA plasma (n=8) 91-104 97
Cell culture media (n=8) 91-107 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 26.40 59.30 201.20 27.30 57.80 199.40
Standard deviation 1.70 3.20 10.10 1.80 2.40 8.80
CV (%) 6.44 5.40 5.02 6.59 4.15 4.41
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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