产品详情

  • 产品名称：Human TSP-2 (Thrombospondin-2) Sandwich ELISA (STJE0019896)

  • 产品型号：STJE0019896
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TSP-2 (Thrombospondin-2) Sandwich ELISA (STJE0019896)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 11.12 pg/mL
Detection Limit: 31.25-2000 pg/mL
Short Description: This TSP-2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: THBS2
Gene ID: 7058
Uniprot ID: TSP2_HUMAN
Specificity: This kit recognizes Human TSP-2 in samples. No significant cross-reactivity or interference between Human TSP-2 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity High expression in invertebral disk tissue.
Function Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
Protein Name Thrombospondin-2
Database Links Reactome: R-HSA-186797
Reactome: R-HSA-5083635
Reactome: R-HSA-5173214
Alternative ELISA Names Thrombospondin-2 ELISA kit
THBS2 ELISA kit
TSP2 ELISA kit
Specificity This kit recognizes Human TSP-2 in samples. No significant cross-reactivity or interference between Human TSP-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-99 91
EDTA plasma (n=8) 90-103 97
Cell culture media (n=8) 91-106 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.02 179.21 933.01 102.00 174.77 988.58
Standard deviation 6.45 8.57 50.10 6.79 9.40 44.19
CV (%) 6.79 4.78 5.37 6.66 5.38 4.47
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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