产品详情

  • 产品名称：Human TERT (Telomerase reverse transcriptase) Sandwich ELISA (STJE0002420)

  • 产品型号：STJE0002420
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TERT (Telomerase reverse transcriptase) Sandwich ELISA (STJE0002420)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This TERT Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TERT
Gene ID: 7015
Uniprot ID: TERT_HUMAN
Specificity: This kit recognizes Human TERT in samples. No significant cross-reactivity or interference between Human TERT and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation at Tyr-707 under oxidative stress leads to translocations of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation at Ser-227 by the AKT pathway promotes nuclear locations. Phosphorylation at the G2/M phase at Ser-457 by DYRK2 promotes ubiquitination by the EDVP complex and degradation. Ubiquitinated by the EDVP complex, a E3 ligase complex following phosphorylation at Ser-457 by DYRK2. Ubiquitinated leads to proteasomal degradation. (Microbial infection) In case of infection by HIV-1, the EDVP complex is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP, leading to ubiquitination and degradation.
Function Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocations followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Protein Name Telomerase Reverse Transcriptase
Hest2
Telomerase Catalytic Subunit
Telomerase-Associated Protein 2
Tp2
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-201722
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Chromosome
Telomere
Cytoplasm
Pml Body
Shuttling Between Nuclear And Cytoplasm Depends On Cell Cycle
Phosphorylation States
Transformation And Dna Damage
Diffuse Localization In The Nucleoplasm
Enriched In Nucleoli Of Certain Cell Types
Translocated To The Cytoplasm Via Nuclear Pores In A Crm1/Ran-Dependent Manner Involving Oxidative Stress-Mediated Phosphorylation At Tyr-707
Dephosphorylation At This Site By Shp2 Retains Tert In The Nucleus
Translocated To The Nucleus By Phosphorylation By Akt
Alternative ELISA Names Telomerase Reverse Transcriptase ELISA kit
Hest2 ELISA kit
Telomerase Catalytic Subunit ELISA kit
Telomerase-Associated Protein 2 ELISA kit
Tp2 ELISA kit
TERT ELISA kit
EST2 ELISA kit
TCS1 ELISA kit
TRT ELISA kit
Specificity This kit recognizes Human TERT in samples. No significant cross-reactivity or interference between Human TERT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 98
EDTA plasma (n=8) 84-98 91
Cell culture media (n=8) 95-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.48 1.58 4.00 0.44 1.58 4.34
Standard deviation 0.03 0.07 0.19 0.02 0.08 0.15
CV (%) 6.25 4.43 4.75 4.55 5.06 3.46
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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