产品详情

  • 产品名称：Human SFTPA1 (Pulmonary surfactant-associated protein A1) Sandwich ELISA (STJE0002837)

  • 产品型号：STJE0002837
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SFTPA1 (Pulmonary surfactant-associated protein A1) Sandwich ELISA (STJE0002837)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This SFTPA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SFTPA1
Gene ID: 653509
Uniprot ID: SFTA1_HUMAN
Specificity: This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages. (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule. Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages. (Microbial infection) Binds M.pneumoniae CARDS toxin, serves as one receptor for this pathogen. When SFTPA1 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
Protein Name Pulmonary Surfactant-Associated Protein A1
Psp-A
Pspa
Sp-A
Sp-A1
35 Kda Pulmonary Surfactant-Associated Protein
Alveolar Proteinosis Protein
Collectin-4
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-168179
Reactome: R-HSA-391160
Reactome: R-HSA-5683826
Reactome: R-HSA-5686938
Reactome: R-HSA-5688849
Reactome: R-HSA-5688890
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Surface Film
Alternative ELISA Names Pulmonary Surfactant-Associated Protein A1 ELISA kit
Psp-A ELISA kit
Pspa ELISA kit
Sp-A ELISA kit
Sp-A1 ELISA kit
35 Kda Pulmonary Surfactant-Associated Protein ELISA kit
Alveolar Proteinosis Protein ELISA kit
Collectin-4 ELISA kit
SFTPA1 ELISA kit
COLEC4 ELISA kit
PSAP ELISA kit
SFTP1 ELISA kit
SFTPA ELISA kit
SFTPA1B ELISA kit
Specificity This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 92
EDTA plasma (n=8) 85-98 91
Cell culture media (n=8) 88-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.86 136.08 414.87 48.73 132.09 424.02
Standard deviation 2.60 7.61 16.18 2.93 5.76 20.01
CV (%) 5.01 5.59 3.90 6.01 4.36 4.72
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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