产品详情

  • 产品名称：Mouse Ccl12 (C-C motif chemokine 12) Sandwich ELISA (STJE0005319)

  • 产品型号：STJE0005319
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Mouse Ccl12 (C-C motif chemokine 12) Sandwich ELISA (STJE0005319)
详情介绍：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Ccl12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ccl12
Gene ID: 20293
Uniprot ID: CCL12_MOUSE
Specificity: This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
Protein Name C-C Motif Chemokine 12
Mcp-1-Related Chemokine
Monocyte Chemoattractant Protein 5
Monocyte Chemotactic Protein 5
Mcp-5
Small-Inducible Cytokine A12
Database Links Reactome: R-MMU-380108
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 12 ELISA kit
Mcp-1-Related Chemokine ELISA kit
Monocyte Chemoattractant Protein 5 ELISA kit
Monocyte Chemotactic Protein 5 ELISA kit
Mcp-5 ELISA kit
Small-Inducible Cytokine A12 ELISA kit
Ccl12 ELISA kit
Mcp5 ELISA kit
Scya12 ELISA kit
Specificity This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma (n=8) 100-110 103
Cell culture media (n=8) 84-96 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 173.22 443.46 1632.31 168.36 471.43 1765.51
Standard deviation 9.16 20.67 72.47 8.96 22.72 91.63
CV (%) 5.29 4.66 4.44 5.32 4.82 5.19
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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