产品详情
简单介绍:
Mouse APC (Activated Protein C) Sandwich ELISA (STJE0005281)
详情介绍:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 46.88pg/mL |
Detection Limit: | 78.13~5000pg/mL |
Short Description: | This APC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Proc |
Gene ID: | 19123 |
Uniprot ID: | PROC_MOUSE |
Specificity: | This kit recognizes Mouse APC in samples. No significant cross-reactivity or interference between Mouse APC and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
Function | Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function. |
Protein Name | Vitamin K-Dependent Protein C Anticoagulant Protein C Autoprothrombin Iia Blood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide |
Database Links | Reactome: R-MMU-140837 Reactome: -MMU-140875 Reactome: -MMU-159740 Reactome: -MMU-159763 Reactome: -MMU-159782 Reactome: -MMU-202733 Reactome: -MMU-381426 Reactome: -MMU-8957275 |
Cellular Localisation | Secreted Golgi Apparatus Endoplasmic Reticulum |
Alternative ELISA Names | Vitamin K-Dependent Protein C ELISA kit Anticoagulant Protein C ELISA kit Autoprothrombin Iia ELISA kit Blood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide ELISA kit Proc ELISA kit |
Specificity | This kit recognizes Mouse APC in samples. No significant cross-reactivity or interference between Mouse APC and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 91-102 | 97 |
EDTA plasma (n=8) | 91-105 | 97 |
Cell culture media (n=8) | 88-100 | 93 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 260.01 | 563.69 | 1988.50 | 274.76 | 561.11 | 1860.49 |
Standard deviation | 16.95 | 32.02 | 97.44 | 16.68 | 32.21 | 74.98 |
CV (%) | 6.52 | 5.68 | 4.90 | 6.07 | 5.74 | 4.03 |
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