产品详情

  • 产品名称：Human Zonulin (Zonulin) Sandwich ELISA (STJE0003933)

  • 产品型号：STJE0003933
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human Zonulin (Zonulin) Sandwich ELISA (STJE0003933)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This Zonulin Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: HP
Gene ID: 3240
Uniprot ID: HPT_HUMAN
Specificity: This kit recognizes Human Zonulin in samples. No significant cross-reactivity or interference between Human Zonulin and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Protein Name Haptoglobin
Zonulin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain
Database Links Reactome: R-HSA-2168880
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative ELISA Names Haptoglobin ELISA kit
Zonulin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain ELISA kit
HP ELISA kit
Specificity This kit recognizes Human Zonulin in samples. No significant cross-reactivity or interference between Human Zonulin and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 95
EDTA plasma (n=8) 87-99 91
Cell culture media (n=8) 91-99 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.46 5.57 22.64 2.31 6.06 23.45
Standard deviation 0.14 0.27 1.23 0.12 0.28 1.02
CV (%) 5.69 4.85 5.43 5.19 4.62 4.35
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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