产品详情

  • 产品名称：Human XOD (Xanthine Oxidase) Sandwich ELISA (STJE0003949)

  • 产品型号：STJE0003949
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human XOD (Xanthine Oxidase) Sandwich ELISA (STJE0003949)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This XOD Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: XDH
Gene ID: 7498
Uniprot ID: XDH_HUMAN
Specificity: This kit recognizes Human XOD in samples. No significant cross-reactivity or interference between Human XOD and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Subject to partial proteolysis.this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro).this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Function Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
Protein Name Xanthine Dehydrogenase/Oxidase Includes - Xanthine Dehydrogenase
Xd - Xanthine Oxidase
Xo
Xanthine Oxidoreductase
Xor
Database Links Reactome: R-HSA-74259
Reactome: R-HSA-8851680
Reactome: R-HSA-9748787
Cellular Localisation Cytoplasm
Peroxisome
Secreted
Alternative ELISA Names Xanthine Dehydrogenase/Oxidase Includes - Xanthine Dehydrogenase ELISA kit
Xd - Xanthine Oxidase ELISA kit
Xo ELISA kit
Xanthine Oxidoreductase ELISA kit
Xor ELISA kit
XDH ELISA kit
XDHA ELISA kit
Specificity This kit recognizes Human XOD in samples. No significant cross-reactivity or interference between Human XOD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 96
EDTA plasma (n=8) 94-106 100
Cell culture media (n=8) 94-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 0.85 4.85 0.49 0.79 4.64
Standard deviation 0.03 0.05 0.22 0.03 0.04 0.25
CV (%) 6.38 5.88 4.54 6.12 5.06 5.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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