产品详情

  • 产品名称：Human TK1 (Thymidine kinase, cytosolic) Sandwich ELISA (STJE0003719)

  • 产品型号：STJE0003719
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TK1 (Thymidine kinase, cytosolic) Sandwich ELISA (STJE0003719)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This TK1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TK1
Gene ID: 7083
Uniprot ID: KITH_HUMAN
Specificity: This kit recognizes Human TK1 in samples. No significant cross-reactivity or interference between Human TK1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cell-cycle-regulated enzyme of importance in nucleotide metabolism. Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate. Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT).
Protein Name Thymidine Kinase - Cytosolic
Database Links Reactome: R-HSA-69205
Reactome: R-HSA-73614
Cellular Localisation Cytoplasm
Alternative ELISA Names Thymidine Kinase - Cytosolic ELISA kit
TK1 ELISA kit
Specificity This kit recognizes Human TK1 in samples. No significant cross-reactivity or interference between Human TK1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-101 93
EDTA plasma (n=8) 85-98 92
Cell culture media (n=8) 91-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 194.44 409.49 1921.23 190.86 404.41 2086.28
Standard deviation 11.96 22.89 102.40 13.32 18.44 76.57
CV (%) 6.15 5.59 5.33 6.98 4.56 3.67
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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