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  • 产品名称：Human THRA (Thyroid hormone receptor alpha) CLIA Kit (STJC0000982)

  • 产品型号：STJC0000982
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human THRA (Thyroid hormone receptor alpha) CLIA Kit (STJC0000982)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human THRA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: THRA
Gene ID: 7067
Uniprot ID: THA_HUMAN
Specificity: This kit recognizes Human THR Alpha in samples. No significant cross-reactivity or interference between Human THR Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Isoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Isoform Alpha-2: Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action.
Protein Name Thyroid Hormone Receptor Alpha
Nuclear Receptor Subfamily 1 Group A Member 1
V-Erba-Related Protein 7
Ear-7
C-Erba-1
C-Erba-Alpha
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294 P10827-2
Cellular Localisation Nucleus
Isoform Alpha-2: Cytoplasm
When Overexpressed Found In The Cytoplasm Where It Colocalizes With Tacc1
Alternative CLIA Names Thyroid Hormone Receptor Alpha CLIA kit
Nuclear Receptor Subfamily 1 Group A Member 1 CLIA kit
V-Erba-Related Protein 7 CLIA kit
Ear-7 CLIA kit
C-Erba-1 CLIA kit
C-Erba-Alpha CLIA kit
THRA CLIA kit
EAR7 CLIA kit
ERBA1 CLIA kit
NR1A1 CLIA kit
THRA1 CLIA kit
THRA2 CLIA kit
Specificity This kit recognizes Human THRα in samples. No significant cross-reactivity or interference between Human THRα and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 92
EDTA plasma(n=8) 93-104 98
Cell culture media(n=8) 95-112 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.34 91.68 364.58 51.82 83.21 342.81
Standard deviation 4.38 8.67 28.66 6.71 7.26 36.99
CV (%) 9.25 9.46 7.86 12.95 8.72 10.79
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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