产品详情

  • 产品名称：Human SERPINF1 (Pigment epithelium-derived factor) CLIA Kit (STJC0000818)

  • 产品型号：STJC0000818
  • 产品厂商：ST john's laboratory
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Human SERPINF1 (Pigment epithelium-derived factor) CLIA Kit (STJC0000818)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human SERPINF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SERPINF1
Gene ID: 5176
Uniprot ID: PEDF_HUMAN
Specificity: This kit recognizes Human PEDF in samples. No significant cross-reactivity or interference between Human PEDF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Retinal pigment epithelial cells and blood plasma.
Post Translational Modifications The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Function Neurotrophic protein.induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Protein Name Pigment Epithelium-Derived Factor
Pedf
Cell Proliferation-Inducing Gene 35 Protein
Epc-1
Serpin F1
Cellular Localisation Secreted
Melanosome
Enriched In Stage I Melanosomes
Alternative CLIA Names Pigment Epithelium-Derived Factor CLIA kit
Pedf CLIA kit
Cell Proliferation-Inducing Gene 35 Protein CLIA kit
Epc-1 CLIA kit
Serpin F1 CLIA kit
SERPINF1 CLIA kit
PEDF CLIA kit
PIG35 CLIA kit
Specificity This kit recognizes Human PEDF in samples. No significant cross-reactivity or interference between Human PEDF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 101-117 107
EDTA plasma(n=8) 95-112 102
Cell culture media(n=8) 92-108 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.67 216.84 818.05 87.30 218.90 884.23
Standard deviation 7.86 20.79 59.15 8.21 16.07 96.29
CV (%) 8.22 9.59 7.23 9.40 7.34 10.89
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
姓名：
电话：
您的需求：
 
网站地图