产品详情

  • 产品名称：Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)

  • 产品型号：STJA0005501-100
  • 产品厂商：ST john's laboratory
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)
详情介绍：
Host: Mouse
Applications: WB/ICC/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-p21/CIP1/WAF1 (Full Length) is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: M513
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:500
IHC 1:50
ICC 1:50
IP 1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN1A
Gene ID: 1026
Uniprot ID: CDN1A_HUMAN
Immunogen Region: Full Length
Specificity: The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC) , HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluores
Immunogen: Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21.
Post Translational Modifications Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocations of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1.
Function Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratioses, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
Protein Name Cyclin-Dependent Kinase Inhibitor 1
Cdk-Interacting Protein 1
Melanoma Differentiation-Associated Protein 6
Mda-6
P21
Database Links Reactome: R-HSA-187577
Reactome: R-HSA-198323
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-5674400
Reactome: R-HSA-6785807
Reactome: R-HSA-6804116
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-69656
Reactome: R-HSA-69895
Reactome: R-HSA-8852276
Reactome: R-HSA-8866911
Reactome: R-HSA-8878166
Reactome: R-HSA-8941855
Reactome: R-HSA-8951664
Reactome: R-HSA-9616222
Reactome: R-HSA-9617828
Reactome: R-HSA-9661069
Reactome: R-HSA-9702518
Reactome: R-HSA-9703465
Reactome: R-HSA-9755511
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Cyclin-Dependent Kinase Inhibitor 1 antibody
Anti-Cdk-Interacting Protein 1 antibody
Anti-Melanoma Differentiation-Associated Protein 6 antibody
Anti-Mda-6 antibody
Anti-P21 antibody
Anti-CDKN1A antibody
Anti-CAP20 antibody
Anti-CDKN1 antibody
Anti-CIP1 antibody
Anti-MDA6 antibody
Anti-PIC1 antibody
Anti-SDI1 antibody
Anti-WAF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
姓名：
电话：
您的需求：
 
网站地图