产品详情

  • 产品名称：Human PRF1 (Perforin-1) Sandwich ELISA (STJE0002736)

  • 产品型号：STJE0002736
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PRF1 (Perforin-1) Sandwich ELISA (STJE0002736)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This PRF1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PRF1
Gene ID: 5551
Uniprot ID: PERF_HUMAN
Specificity: This kit recognizes Human PRF1 in samples. No significant cross-reactivity or interference between Human PRF1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Pfp
Cellular Localisation Cytolytic Granule
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative ELISA Names Perforin-1 ELISA kit
P1 ELISA kit
Cytolysin ELISA kit
Lymphocyte Pore-Forming Protein ELISA kit
Pfp ELISA kit
PRF1 ELISA kit
PFP ELISA kit
Specificity This kit recognizes Human PRF1 in samples. No significant cross-reactivity or interference between Human PRF1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 94-112 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.70 6.61 23.43 2.46 7.19 21.35
Standard deviation 0.18 0.30 0.98 0.17 0.42 1.06
CV (%) 6.67 4.54 4.18 6.91 5.84 4.96
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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