产品详情

  • 产品名称：Anti-CDC42 antibody (118-191) (STJ29840)

  • 产品型号：STJ29840
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Anti-CDC42 antibody (118-191) (STJ29840)
详情介绍：
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDC42 (118-191) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC42
Gene ID: 998
Uniprot ID: CDC42_HUMAN
Immunogen Region: 118-191
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 118-191 of human CDC42 (NP_001782.1).
Immunogen Sequence: DLRDDPSTIEKLAKNKQKPI TPETAEKLARDLKAVKYVEC SALTQKGLKNVFDEAILAAL EPPEPKKSRRCVLL
Post Translational Modifications (Microbial infection) AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation.FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI. (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of CDC42 and leads to actin disassembly. (Microbial infection) Glucosylated at Thr-35 by C.difficile toxins TcdA and TcdB in the colonic epithelium. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function. (Microbial infection) Glycosylated (O-GlcNAcylated) at Thr-35 by C.novyi toxin TcdA. O-GlcNAcylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption.
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
Protein Name Cell Division Control Protein 42 Homolog
G25k Gtp-Binding Protein
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-182971
Reactome: R-HSA-2029482
Reactome: R-HSA-389359
Reactome: R-HSA-3928662
Reactome: R-HSA-418885
Reactome: R-HSA-428543
Reactome: R-HSA-4420097
Reactome: R-HSA-525793
Reactome: R-HSA-5625970
Reactome: R-HSA-5626467
Reactome: R-HSA-5627123
Reactome: R-HSA-5663213
Reactome: R-HSA-5663220
Reactome: R-HSA-5687128
Reactome: R-HSA-8950505
Reactome: R-HSA-8964616
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Reactome: R-HSA-9013423
Reactome: R-HSA-9013424
Reactome: R-HSA-9664422
Reactome: R-HSA-983231
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Midbody
Cell Projection
Dendrite
Localizes To Spindle During Prometaphase Cells
Moves To The Central Spindle As Cells Progressed Through Anaphase To Telophase
Localizes At The End Of Cytokinesis In The Intercellular Bridge Formed Between Two Daughter Cells
Its Localization Is Regulated By The Activities Of Guanine Nucleotide Exchange Factor Ect2 And Gtpase Activating Protein Racgap1
Colocalizes With Nek6 In The Centrosome
In Its Active Gtp-Bound Form Localizes To The Leading Edge Membrane Of Migrating Dendritic Cells
Alternative Antibody Names Anti-Cell Division Control Protein 42 Homolog antibody
Anti-G25k Gtp-Binding Protein antibody
Anti-CDC42 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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