产品详情

  • 产品名称：Human PON1 (Serum paraoxonase/arylesterase 1) CLIA Kit (STJC0000942)

  • 产品型号：STJC0000942
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PON1 (Serum paraoxonase/arylesterase 1) CLIA Kit (STJC0000942)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human PON1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: PON1
Gene ID: 5444
Uniprot ID: PON1_HUMAN
Specificity: This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
Post Translational Modifications Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not.
Function Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Protein Name Serum Paraoxonase/Arylesterase 1
Pon 1
Aromatic Esterase 1
A-Esterase 1
K-45
Serum Aryldialkylphosphatase 1
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-9754706
Cellular Localisation Secreted
Extracellular Space
Alternative CLIA Names Serum Paraoxonase/Arylesterase 1 CLIA kit
Pon 1 CLIA kit
Aromatic Esterase 1 CLIA kit
A-Esterase 1 CLIA kit
K-45 CLIA kit
Serum Aryldialkylphosphatase 1 CLIA kit
PON1 CLIA kit
PON CLIA kit
Specificity This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-114 107
EDTA plasma(n=8) 84-98 91
Cell culture media(n=8) 95-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 101.79 198.63 730.05 108.67 207.99 757.05
Standard deviation 8.54 22.45 52.05 12.40 24.15 70.48
CV (%) 8.39 11.30 7.13 11.41 11.61 9.31
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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