产品详情
简单介绍：
Human POMC (Pro-opiomelanocortin) Competitive ELISA (STJE0002010)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This POMC Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 2.5h |
| Detection: | Colormetric |
| Gene Symbol: | POMC |
| Gene ID: | 5443 |
| Uniprot ID: | COLI_HUMAN |
| Specificity: | This kit recognizes Human ACTH in samples. No significant cross-reactivity or interference between Human ACTH and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated.reducing sugar is probably N-acetylgalactosamine. |
| Function | Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate. |
| Protein Name | Pro-Opiomelanocortin Pomc Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma Gamma-Msh - Potential Peptide - Corticotropin Adrenocorticotropic Hormone Acth - Melanocyte-Stimulating Hormone Alpha Alpha-Msh Melanotropin Alpha - Corticotropin-Like Intermediary Peptide Clip - Lipotropin Beta Beta-Lph - Lipotropin Gamma Gamma-Lph - Melanocyte-Stimulating Hormone Beta Beta-Msh Melanotropin Beta - Beta-Endorphin - Met-Enkephalin |
| Database Links | Reactome: R-HSA-111885 Reactome: R-HSA-193048 Reactome: R-HSA-194002 Reactome: R-HSA-202040 Reactome: R-HSA-209952 Reactome: R-HSA-211976 Reactome: R-HSA-375276 Reactome: R-HSA-418555 Reactome: R-HSA-418594 Reactome: R-HSA-5579031 Reactome: R-HSA-6785807 Reactome: R-HSA-9615017 |
| Cellular Localisation | Secreted Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms |
| Alternative ELISA Names | Pro-Opiomelanocortin ELISA kit Pomc ELISA kit Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma ELISA kit Gamma-Msh - Potential Peptide - Corticotropin ELISA kit Adrenocorticotropic Hormone ELISA kit Acth - Melanocyte-Stimulating Hormone Alpha ELISA kit Alpha-Msh ELISA kit Melanotropin Alpha - Corticotropin-Like Intermediary Peptide ELISA kit Clip - Lipotropin Beta ELISA kit Beta-Lph - Lipotropin Gamma ELISA kit Gamma-Lph - Melanocyte-Stimulating Hormone Beta ELISA kit Beta-Msh ELISA kit Melanotropin Beta - Beta-Endorphin - Met-Enkephalin ELISA kit POMC ELISA kit |
| Specificity | This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 91-104 | 98 |
| EDTA plasma(n=8) | 85-100 | 91 |
| Cell culture media(n=8) | 86-98 | 93 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 45.70 | 130.90 | 461.60 | 45.10 | 124.70 | 448.60 |
| Standard deviation | 3.20 | 6.80 | 14.30 | 2.30 | 6.40 | 19.70 |
| CV (%) | 7.00 | 5.19 | 3.10 | 5.10 | 5.13 | 4.39 |
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