产品详情

  • 产品名称：Human POMC (Pro-opiomelanocortin) CLIA Kit (STJC0000491)

  • 产品型号：STJC0000491
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human POMC (Pro-opiomelanocortin) CLIA Kit (STJC0000491)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human POMC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: POMC
Gene ID: 5443
Uniprot ID: COLI_HUMAN
Specificity: This kit recognizes Human POMC in samples. No significant cross-reactivity or interference between Human POMC and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity ACTH and MSH are produced by the pituitary gland.
Post Translational Modifications Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated.reducing sugar is probably N-acetylgalactosamine.
Function Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate.
Protein Name Pro-Opiomelanocortin
Pomc
Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma
Gamma-Msh - Potential Peptide - Corticotropin
Adrenocorticotropic Hormone
Acth - Melanocyte-Stimulating Hormone Alpha
Alpha-Msh
Melanotropin Alpha - Corticotropin-Like Intermediary Peptide
Clip - Lipotropin Beta
Beta-Lph - Lipotropin Gamma
Gamma-Lph - Melanocyte-Stimulating Hormone Beta
Beta-Msh
Melanotropin Beta - Beta-Endorphin - Met-Enkephalin
Database Links Reactome: R-HSA-111885
Reactome: R-HSA-193048
Reactome: R-HSA-194002
Reactome: R-HSA-202040
Reactome: R-HSA-209952
Reactome: R-HSA-211976
Reactome: R-HSA-375276
Reactome: R-HSA-418555
Reactome: R-HSA-418594
Reactome: R-HSA-5579031
Reactome: R-HSA-6785807
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons
Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms
Alternative CLIA Names Pro-Opiomelanocortin CLIA kit
Pomc CLIA kit
Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma CLIA kit
Gamma-Msh - Potential Peptide - Corticotropin CLIA kit
Adrenocorticotropic Hormone CLIA kit
Acth - Melanocyte-Stimulating Hormone Alpha CLIA kit
Alpha-Msh CLIA kit
Melanotropin Alpha - Corticotropin-Like Intermediary Peptide CLIA kit
Clip - Lipotropin Beta CLIA kit
Beta-Lph - Lipotropin Gamma CLIA kit
Gamma-Lph - Melanocyte-Stimulating Hormone Beta CLIA kit
Beta-Msh CLIA kit
Melanotropin Beta - Beta-Endorphin - Met-Enkephalin CLIA kit
POMC CLIA kit
Specificity This kit recognizes Human POMC in samples. No significant cross-reactivity or interference between Human POMC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 97
EDTA plasma(n=8) 94-107 101
Cell culture media(n=8) 90-105 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 111.10 236.43 880.16 117.42 226.88 807.21
Standard deviation 10.65 20.83 68.21 13.94 19.49 81.85
CV (%) 9.59 8.81 7.75 11.87 8.59 10.14
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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