产品详情

  • 产品名称：Human PODXL (Podocalyxin) CLIA Kit (STJC0000957)

  • 产品型号：STJC0000957
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PODXL (Podocalyxin) CLIA Kit (STJC0000957)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human PODXL kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: PODXL
Gene ID: 5420
Uniprot ID: PODXL_HUMAN
Specificity: This kit recognizes Human PCX in samples. No significant cross-reactivity or interference between Human PCX and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Glomerular epithelium cell (podocyte).
Post Translational Modifications N- and O-linked glycosylated. Sialoglycoprotein.
Function Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Protein Name Podocalyxin
Gctm-2 Antigen
Gp200
Podocalyxin-Like Protein 1
Pc
Pclp-1
Cellular Localisation Apical Cell Membrane
Cell Projection
Lamellipodsium
Filopodium
Ruffle
Microvillus
Membrane Raft
Membrane
Single-Pass Type I Membrane Protein
In Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet Polarized
Colocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial Polarization
Colocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma Membrane
Its Association With The Membrane Raft Is Transient
Colocalizes With Actin Filaments
Ezr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli Form
Colocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium Cells
Forms Granular
Punctuated Pattern
Forming Patches
Preferentially Adopting A Polar Distribution
Located On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodsia Establishing Contact With The Endothelial Cells
Colocalizes With The Submembrane Actin Of Lamellipodsia
Particularly Associated With Ruffles
Colocalizes With Vinculin At Protrusions Of Cells
Colocalizes With Itgb1
Colocalizes With Pard3
Prkci
Exoc5
Ocln
Rab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis
Alternative CLIA Names Podocalyxin CLIA kit
Gctm-2 Antigen CLIA kit
Gp200 CLIA kit
Podocalyxin-Like Protein 1 CLIA kit
Pc CLIA kit
Pclp-1 CLIA kit
PODXL CLIA kit
PCLP CLIA kit
PCLP1 CLIA kit
Specificity This kit recognizes Human PCX in samples. No significant cross-reactivity or interference between Human PCX and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 94
EDTA plasma(n=8) 89-102 94
Cell culture media(n=8) 93-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 105.27 300.96 813.39 111.32 312.48 741.42
Standard deviation 12.13 22.63 57.51 13.60 28.00 73.55
CV (%) 11.52 7.52 7.07 12.22 8.96 9.92
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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