产品详情

  • 产品名称：Human NPHS2 (Podocin) Sandwich ELISA (STJE0002580)

  • 产品型号：STJE0002580
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human NPHS2 (Podocin) Sandwich ELISA (STJE0002580)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This NPHS2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: NPHS2
Gene ID: 7827
Uniprot ID: PODO_HUMAN
Specificity: This kit recognizes Human PDCN in samples. No significant cross-reactivity or interference between Human PDCN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Isoform 2: Glycosylated.
Function Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Protein Name Podocin
Database Links Reactome: R-HSA-373753
Cellular Localisation Isoform 1: Cell Membrane
Peripheral Membrane Protein
Isoform 2: Endoplasmic Reticulum
Alternative ELISA Names Podocin ELISA kit
NPHS2 ELISA kit
Specificity This kit recognizes Human PDCN in samples. No significant cross-reactivity or interference between Human PDCN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 100
EDTA plasma (n=8) 87-102 93
Cell culture media (n=8) 95-109 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.09 2.58 7.62 1.13 2.48 7.34
Standard deviation 0.07 0.12 0.33 0.06 0.14 0.27
CV (%) 6.42 4.65 4.33 5.31 5.65 3.68
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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