产品详情

  • 产品名称：Human MGLL (Monoglyceride lipase) CLIA Kit (STJC0000301)

  • 产品型号：STJC0000301
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MGLL (Monoglyceride lipase) CLIA Kit (STJC0000301)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human MGLL kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: MGLL
Gene ID: 11343
Uniprot ID: MGLL_HUMAN
Specificity: This kit recognizes Human MGL in samples. No significant cross-reactivity or interference between Human MGL and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in adipose tissue, lung, liver, kidney, brain and heart.
Function Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
Protein Name Monoglyceride Lipase
Mgl
Hu-K5
Lysophospholipase Homolog
Lysophospholipase-Like
Monoacylglycerol Lipase
Magl
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-163560
Reactome: R-HSA-426048
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative CLIA Names Monoglyceride Lipase CLIA kit
Mgl CLIA kit
Hu-K5 CLIA kit
Lysophospholipase Homolog CLIA kit
Lysophospholipase-Like CLIA kit
Monoacylglycerol Lipase CLIA kit
Magl CLIA kit
MGLL CLIA kit
Specificity This kit recognizes Human MGL in samples. No significant cross-reactivity or interference between Human MGL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 98
EDTA plasma(n=8) 93-106 100
Cell culture media(n=8) 93-110 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.03 290.16 822.18 86.07 280.94 743.11
Standard deviation 7.66 23.94 71.04 8.47 32.28 74.39
CV (%) 8.06 8.25 8.64 9.84 11.49 10.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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