产品详情

  • 产品名称：Human MBP-C/MBL2 (Mannose-binding protein C) Sandwich ELISA (STJE0002870)

  • 产品型号：STJE0002870
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MBP-C/MBL2 (Mannose-binding protein C) Sandwich ELISA (STJE0002870)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This MBP-C/MBL2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MBL2
Gene ID: 4153
Uniprot ID: MBL2_HUMAN
Specificity: This kit recognizes Human MBP-C/MBL2 in samples. No significant cross-reactivity or interference between Human MBP-C/MBL2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response.
Protein Name Mannose-Binding Protein C
Mbp-C
Collectin-1
Mbp1
Mannan-Binding Protein
Mannose-Binding Lectin
Database Links Reactome: R-HSA-166662
Reactome: R-HSA-166663
Reactome: R-HSA-9705671
Cellular Localisation Secreted
Alternative ELISA Names Mannose-Binding Protein C ELISA kit
Mbp-C ELISA kit
Collectin-1 ELISA kit
Mbp1 ELISA kit
Mannan-Binding Protein ELISA kit
Mannose-Binding Lectin ELISA kit
MBL2 ELISA kit
COLEC1 ELISA kit
MBL ELISA kit
Specificity This kit recognizes Human MBP-C/MBL2 in samples. No significant cross-reactivity or interference between Human MBP-C/MBL2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 91
EDTA plasma (n=8) 86-100 92
Cell culture media (n=8) 87-101 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.52 1.58 4.58 0.52 1.59 5.01
Standard deviation 0.03 0.07 0.23 0.03 0.07 0.17
CV (%) 5.77 4.43 5.02 5.77 4.40 3.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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