产品详情

  • 产品名称：Human LGALS8 (Galectin-8) CLIA Kit (STJC0000506)

  • 产品型号：STJC0000506
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human LGALS8 (Galectin-8) CLIA Kit (STJC0000506)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human LGALS8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: LGALS8
Gene ID: 3964
Uniprot ID: LEG8_HUMAN
Specificity: This kit recognizes Human GAL8 in samples. No significant cross-reactivity or interference between Human GAL8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Function Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane.these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.
Protein Name Galectin-8
Gal-8
Po66 Carbohydrate-Binding Protein
Po66-Cbp
Prostate Carcinoma Tumor Antigen 1
Pcta-1
Cellular Localisation Cytoplasmic Vesicle
Cytoplasm
Cytosol
Alternative CLIA Names Galectin-8 CLIA kit
Gal-8 CLIA kit
Po66 Carbohydrate-Binding Protein CLIA kit
Po66-Cbp CLIA kit
Prostate Carcinoma Tumor Antigen 1 CLIA kit
Pcta-1 CLIA kit
LGALS8 CLIA kit
Specificity This kit recognizes Human GAL8 in samples. No significant cross-reactivity or interference between Human GAL8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-105 96
EDTA plasma(n=8) 89-103 94
Cell culture media(n=8) 89-101 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 191.34 463.25 1596.46 179.17 475.67 1504.06
Standard deviation 24.26 41.09 137.93 18.63 47.61 172.82
CV (%) 12.68 8.87 8.64 10.40 10.01 11.49
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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