产品详情

  • 产品名称：Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA (STJE0003429)

  • 产品型号：STJE0003429
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA (STJE0003429)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.75mIU/mL
Detection Limit: 6.25~400mIU/mL
Short Description: This KRT18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: KRT18
Gene ID: 3875
Uniprot ID: K1C18_HUMAN
Specificity: This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name Keratin - Type I Cytoskeletal 18
Cell Proliferation-Inducing Gene 46 Protein
Cytokeratin-18
Ck-18
Keratin-18
K18
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Nucleus Matrix
Cytoplasm
Perinuclear Region
Nucleus
Nucleolus
Alternative ELISA Names Keratin - Type I Cytoskeletal 18 ELISA kit
Cell Proliferation-Inducing Gene 46 Protein ELISA kit
Cytokeratin-18 ELISA kit
Ck-18 ELISA kit
Keratin-18 ELISA kit
K18 ELISA kit
KRT18 ELISA kit
CYK18 ELISA kit
PIG46 ELISA kit
Specificity This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 99
EDTA plasma (n=8) 95-107 100
Cell culture media (n=8) 85-100 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (mIU/mL) 18.51 61.86 159.46 19.61 63.54 150.53
Standard deviation 1.12 2.50 7.35 1.13 3.18 5.81
CV (%) 6.05 4.04 4.61 5.76 5.00 3.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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