产品详情

  • 产品名称：Human KLK7 (Kallikrein-7) Sandwich ELISA (STJE0002271)

  • 产品型号：STJE0002271
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human KLK7 (Kallikrein-7) Sandwich ELISA (STJE0002271)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This KLK7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: KLK7
Gene ID: 5650
Uniprot ID: KLK7_HUMAN
Specificity: This kit recognizes Human KLK7 in samples. No significant cross-reactivity or interference between Human KLK7 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
Protein Name Kallikrein-7
Hk7
Serine Protease 6
Stratum Corneum Chymotryptic Enzyme
Hscce
Database Links Reactome: R-HSA-1474228
Cellular Localisation Secreted
In Ovarian Carcinoma
Secreted And Also Observed At The Apical Membrane And In Cytoplasm At The Invasive Front
Alternative ELISA Names Kallikrein-7 ELISA kit
Hk7 ELISA kit
Serine Protease 6 ELISA kit
Stratum Corneum Chymotryptic Enzyme ELISA kit
Hscce ELISA kit
KLK7 ELISA kit
PRSS6 ELISA kit
SCCE ELISA kit
Specificity This kit recognizes Human KLK7 in samples. No significant cross-reactivity or interference between Human KLK7 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 97
EDTA plasma (n=8) 90-102 95
Cell culture media (n=8) 85-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.38 3.62 0.44 1.38 3.58
Standard deviation 0.03 0.07 0.11 0.02 0.07 0.15
CV (%) 6.52 5.07 3.04 4.55 5.07 4.19
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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