产品详情

  • 产品名称：Human IL4I1 (L-amino-acid oxidase) Sandwich ELISA (STJE0003592)

  • 产品型号：STJE0003592
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL4I1 (L-amino-acid oxidase) Sandwich ELISA (STJE0003592)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This IL4I1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL4I1
Gene ID: 259307
Uniprot ID: OXLA_HUMAN
Specificity: This kit recognizes Human IL4I1 in samples. No significant cross-reactivity or interference between Human IL4I1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated.
Function Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
Protein Name L-Amino-Acid Oxidase
Laao
Lao
Interleukin-4-Induced Protein 1
Il4-Induced Protein 1
Hil4i1
Protein Fig-1
Hfig1
Database Links Reactome: R-HSA-8964208
Cellular Localisation Secreted
Lysosome
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Secreted At The Immunological Synapse
Alternative ELISA Names L-Amino-Acid Oxidase ELISA kit
Laao ELISA kit
Lao ELISA kit
Interleukin-4-Induced Protein 1 ELISA kit
Il4-Induced Protein 1 ELISA kit
Hil4i1 ELISA kit
Protein Fig-1 ELISA kit
Hfig1 ELISA kit
IL4I1 ELISA kit
FIG1 ELISA kit
UNQ636 ELISA kit
PRO1265 ELISA kit
Specificity This kit recognizes Human IL4I1 in samples. No significant cross-reactivity or interference between Human IL4I1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 91
EDTA plasma (n=8) 89-102 96
Cell culture media (n=8) 86-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.56 4.00 0.52 1.47 4.19
Standard deviation 0.03 0.07 0.15 0.03 0.06 0.20
CV (%) 6.00 4.49 3.75 5.77 4.08 4.77
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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