产品详情

  • 产品名称：Human IGF1 (Insulin-like growth factor I) Sandwich ELISA (STJE0006753)

  • 产品型号：STJE0006753
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IGF1 (Insulin-like growth factor I) Sandwich ELISA (STJE0006753)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.01ng/mL
Detection Limit: 3.12-100ng/mL
Short Description: This IGF1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual.
Assay Time: 1.5h
Detection: Colormetric
Gene Symbol: IGF1
Gene ID: 3479
Uniprot ID: IGF1_HUMAN
Specificity: This kit recognizes Human IGF1 in samples. No significant cross-reactivity or interference between Human IGF1 and analogues was observed.
Sample Type: Serum and plasma
Protein Name Insulin-Like Growth Factor I
Igf-I
Mechano Growth Factor
Mgf
Somatomedin-C
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2404192
Reactome: R-HSA-2428928
Reactome: R-HSA-2428933
Reactome: R-HSA-381426
Reactome: R-HSA-422085
Cellular Localisation Secreted
Alternative ELISA Names Insulin-Like Growth Factor I ELISA kit
Igf-I ELISA kit
Mechano Growth Factor ELISA kit
Mgf ELISA kit
Somatomedin-C ELISA kit
IGF1 ELISA kit
IBP1 ELISA kit
Specificity This kit recognizes IGF-1 in samples. No significant cross-reactivity or interference between IGF-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T 2-8℃, 1 months
Reference Standard 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×) 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL 2-8℃ (Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×) 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃ (Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma(n=8) 90-104 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 108.85 204.73 939.71 108.24 188.86 876.39
Standard deviation 6.29 9.87 28.76 6.30 11.05 41.80
CV (%) 5.78 4.82 3.06 5.82 5.85 4.77
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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