产品详情

  • 产品名称：Human HYOU1 (Hypoxia up-regulated protein 1) CLIA Kit (STJC0000250)

  • 产品型号：STJC0000250
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human HYOU1 (Hypoxia up-regulated protein 1) CLIA Kit (STJC0000250)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human HYOU1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: HYOU1
Gene ID: 10525
Uniprot ID: HYOU1_HUMAN
Specificity: This kit recognizes Human HYOU1 in samples. No significant cross-reactivity or interference between Human HYOU1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
Function Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
Protein Name Hypoxia Up-Regulated Protein 1
150 Kda Oxygen-Regulated Protein
Orp-150
170 Kda Glucose-Regulated Protein
Grp-170
Database Links Reactome: R-HSA-3000484
Reactome: R-HSA-381038
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative CLIA Names Hypoxia Up-Regulated Protein 1 CLIA kit
150 Kda Oxygen-Regulated Protein CLIA kit
Orp-150 CLIA kit
170 Kda Glucose-Regulated Protein CLIA kit
Grp-170 CLIA kit
HYOU1 CLIA kit
GRP170 CLIA kit
ORP150 CLIA kit
Specificity This kit recognizes Human HYOU1 in samples. No significant cross-reactivity or interference between Human HYOU1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma(n=8) 98-112 104
Cell culture media(n=8) 88-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 92.55 208.06 994.36 91.87 214.60 923.19
Standard deviation 11.22 17.81 111.67 9.52 24.68 92.78
CV (%) 12.12 8.56 11.23 10.36 11.50 10.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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