产品详情

  • 产品名称：Human FGFR4 (Fibroblast growth factor receptor 4) Sandwich ELISA (STJE0004047)

  • 产品型号：STJE0004047
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human FGFR4 (Fibroblast growth factor receptor 4) Sandwich ELISA (STJE0004047)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This FGFR4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: FGFR4
Gene ID: 2264
Uniprot ID: FGFR4_HUMAN
Specificity: This kit recognizes Human FGFR4 in samples. No significant cross-reactivity or interference between Human FGFR4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19. Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated. Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.
Function Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosesynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation.MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
Protein Name Fibroblast Growth Factor Receptor 4
Fgfr-4
Cd Antigen Cd334
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1307965
Reactome: R-HSA-1839128
Reactome: R-HSA-190322
Reactome: R-HSA-2219530
Reactome: R-HSA-5654228
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654733
Reactome: R-HSA-5655291
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endosome
Endoplasmic Reticulum
Internalized From The Cell Membrane To Recycling Endosomes
And From There Back To The Cell Membrane
Isoform 2: Secreted
Alternative ELISA Names Fibroblast Growth Factor Receptor 4 ELISA kit
Fgfr-4 ELISA kit
Cd Antigen Cd334 ELISA kit
FGFR4 ELISA kit
JTK2 ELISA kit
TKF ELISA kit
Specificity This kit recognizes Human FGFR4 in samples. No significant cross-reactivity or interference between Human FGFR4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 100
EDTA plasma (n=8) 87-98 94
Cell culture media (n=8) 90-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 247.66 595.52 2334.42 245.13 539.87 2151.33
Standard deviation 13.60 29.30 105.52 14.36 24.94 107.57
CV (%) 5.49 4.92 4.52 5.86 4.62 5.00
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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