产品详情

  • 产品名称：Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958)

  • 产品型号：STJE0001958
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 2.35pg/mL
Detection Limit: 3.91~250pg/mL
Short Description: This EGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: EGF
Gene ID: 1950
Uniprot ID: EGF_HUMAN
Specificity: This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
Protein Name Pro-Epidermal Growth Factor
Egf Cleaved Into - Epidermal Growth Factor
Urogastrone
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1227986
Reactome: R-HSA-1236382
Reactome: R-HSA-1236394
Reactome: R-HSA-1250196
Reactome: R-HSA-1251932
Reactome: R-HSA-1257604
Reactome: R-HSA-177929
Reactome: R-HSA-179812
Reactome: R-HSA-180292
Reactome: R-HSA-180336
Reactome: R-HSA-182971
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-212718
Reactome: R-HSA-2219530
Reactome: R-HSA-5637810
Reactome: R-HSA-5638303
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8863795
Reactome: R-HSA-9009391
Reactome: R-HSA-9013507
Reactome: R-HSA-9634638
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Pro-Epidermal Growth Factor ELISA kit
Egf Cleaved Into - Epidermal Growth Factor ELISA kit
Urogastrone ELISA kit
EGF ELISA kit
Specificity This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 92
EDTA plasma (n=8) 90-104 95
Cell culture media (n=8) 90-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 13.20 24.10 114.10 13.00 25.80 123.20
Standard deviation 0.70 1.30 5.90 0.70 1.50 3.80
CV (%) 5.30 5.39 5.17 5.38 5.81 3.08
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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