产品详情

  • 产品名称：Human DNAJB1 (DnaJ homolog subfamily B member 1) CLIA Kit (STJC0000810)

  • 产品型号：STJC0000810
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human DNAJB1 (DnaJ homolog subfamily B member 1) CLIA Kit (STJC0000810)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human DNAJB1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: DNAJB1
Gene ID: 3337
Uniprot ID: DNJB1_HUMAN
Specificity: This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).
Protein Name Dnaj Homolog Subfamily B Member 1
Dnaj Protein Homolog 1
Heat Shock 40 Kda Protein 1
Hsp40
Heat Shock Protein 40
Human Dnaj Protein 1
Hdj-1
Database Links Reactome: R-HSA-3371453
Reactome: R-HSA-3371497
Reactome: R-HSA-3371568
Reactome: R-HSA-3371571
Reactome: R-HSA-5687128
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Translocates Rapidly From The Cytoplasm To The Nucleus
And Especially To The Nucleoli
Upon Heat Shock
Alternative CLIA Names Dnaj Homolog Subfamily B Member 1 CLIA kit
Dnaj Protein Homolog 1 CLIA kit
Heat Shock 40 Kda Protein 1 CLIA kit
Hsp40 CLIA kit
Heat Shock Protein 40 CLIA kit
Human Dnaj Protein 1 CLIA kit
Hdj-1 CLIA kit
DNAJB1 CLIA kit
DNAJ1 CLIA kit
HDJ1 CLIA kit
HSPF1 CLIA kit
Specificity This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 100
EDTA plasma(n=8) 85-99 92
Cell culture media(n=8) 92-104 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 104.10 176.93 877.45 99.93 193.57 797.78
Standard deviation 9.47 14.90 83.80 12.28 23.17 48.51
CV (%) 9.10 8.42 9.55 12.29 11.97 6.08
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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