产品详情

  • 产品名称：Human DHEA-S (Dehydroepiandrosterone Sulfate) Competitive ELISA (STJE0003395)

  • 产品型号：STJE0003395
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human DHEA-S (Dehydroepiandrosterone Sulfate) Competitive ELISA (STJE0003395)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38ng/mL
Detection Limit: 15.63~1000ng/mL
Short Description: This DHEA-S Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Specificity: This kit recognizes Human DHEA-S in samples. No significant cross-reactivity or interference between Human DHEA-S and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Background This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DHEA-S. During the reaction, Human DHEA-S in the sample or standard competes with a fixed amount of Human DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DHEA-S. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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