产品详情

  • 产品名称：Human CXCL8 (Interleukin-8) Sandwich ELISA (STJE0006755)

  • 产品型号：STJE0006755
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CXCL8 (Interleukin-8) Sandwich ELISA (STJE0006755)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63-1000pg/mL
Short Description: This CXCL8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual.
Assay Time: 1.5h
Detection: Colormetric
Gene Symbol: CXCL8
Gene ID: 3576
Uniprot ID: IL8_HUMAN
Specificity: This kit recognizes Human IL8 in samples. No significant cross-reactivity or interference between Human IL8 and analogues was observed.
Sample Type: Serum and plasma
Function Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways.
Protein Name Interleukin-8
Il-8
C-X-C Motif Chemokine 8
Chemokine
C-X-C Motif Ligand 8
Emoctakin
Granulocyte Chemotactic Protein 1
Gcp-1
Monocyte-Derived Neutrophil Chemotactic Factor
Mdncf
Monocyte-Derived Neutrophil-Activating Peptide
Monap
Neutrophil-Activating Protein 1
Nap-1
Protein 3-10c
T-Cell Chemotactic Factor Cleaved Into - Mdncf-A
Gcp/Il-8 Protein Iv
Il8/Nap1 Form I - Interleukin-8
(Ala-Il-877
Gcp/Il-8 Protein Ii
Il-8(1-77
Il8/Nap1 Form Ii
Mdncf-B - Il-8(5-77 - Il-8(6-77
(Ser-Il-872
Gcp/Il-8 Protein I
Il8/Nap1 Form Iii
Lymphocyte-Derived Neutrophil-Activating Factor
Lynap
Mdncf-C
Neutrophil-Activating Factor
Naf - Il-8(7-77
Gcp/Il-8 Protein V
Il8/Nap1 Form Iv - Il-8(8-77
Gcp/Il-8 Protein Vi
Il8/Nap1 Form V - Il-8(9-77
Gcp/Il-8 Protein Iii
Il8/Nap1 Form Vi
Database Links Reactome: R-HSA-2559582
Reactome: R-HSA-375276
Reactome: R-HSA-380108
Reactome: R-HSA-380994
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-8 ELISA kit
Il-8 ELISA kit
C-X-C Motif Chemokine 8 ELISA kit
Chemokine ELISA kit
C-X-C Motif Ligand 8 ELISA kit
Emoctakin ELISA kit
Granulocyte Chemotactic Protein 1 ELISA kit
Gcp-1 ELISA kit
Monocyte-Derived Neutrophil Chemotactic Factor ELISA kit
Mdncf ELISA kit
Monocyte-Derived Neutrophil-Activating Peptide ELISA kit
Monap ELISA kit
Neutrophil-Activating Protein 1 ELISA kit
Nap-1 ELISA kit
Protein 3-10c ELISA kit
T-Cell Chemotactic Factor Cleaved Into - Mdncf-A ELISA kit
Gcp/Il-8 Protein Iv ELISA kit
Il8/Nap1 Form I - Interleukin-8 ELISA kit
(Ala-Il-877 ELISA kit
Gcp/Il-8 Protein Ii ELISA kit
Il-8(1-77 ELISA kit
Il8/Nap1 Form Ii ELISA kit
Mdncf-B - Il-8(5-77 - Il-8(6-77 ELISA kit
(Ser-Il-872 ELISA kit
Gcp/Il-8 Protein I ELISA kit
Il8/Nap1 Form Iii ELISA kit
Lymphocyte-Derived Neutrophil-Activating Factor ELISA kit
Lynap ELISA kit
Mdncf-C ELISA kit
Neutrophil-Activating Factor ELISA kit
Naf - Il-8(7-77 ELISA kit
Gcp/Il-8 Protein V ELISA kit
Il8/Nap1 Form Iv - Il-8(8-77 ELISA kit
Gcp/Il-8 Protein Vi ELISA kit
Il8/Nap1 Form V - Il-8(9-77 ELISA kit
Gcp/Il-8 Protein Iii ELISA kit
Il8/Nap1 Form Vi ELISA kit
CXCL8 ELISA kit
IL8 ELISA kit
Specificity This kit recognizes IL-8 in samples. No significant cross-reactivity or interference between IL-8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T 2-8℃, 1 months
Reference Standard 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×) 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL 2-8℃ (Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×) 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃ (Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 97
EDTA plasma(n=8) 86-97 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.94 152.77 425.52 55.10 154.08 436.69
Standard deviation 3.28 6.97 16.13 3.07 8.67 14.89
CV (%) 5.97 4.56 3.79 5.58 5.63 3.41
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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