产品详情

  • 产品名称：Human CP (Ceruloplasmin) Sandwich ELISA (STJE0004038)

  • 产品型号：STJE0004038
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CP (Ceruloplasmin) Sandwich ELISA (STJE0004038)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47 Mu g/mL
Detection Limit: 0.78~50 Mu g/mL
Short Description: This CP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CP
Gene ID: 1356
Uniprot ID: CERU_HUMAN
Specificity: This kit recognizes Human CP in samples. No significant cross-reactivity or interference between Human CP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense.
Protein Name Ceruloplasmin
Ferroxidase
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-425410
Reactome: R-HSA-5619049
Reactome: R-HSA-5619060
Reactome: R-HSA-8957275
Reactome: R-HSA-917937
Cellular Localisation Secreted
Colocalizes With Gcp1 In Secretory Intracellular Compartments
Alternative ELISA Names Ceruloplasmin ELISA kit
Ferroxidase ELISA kit
CP ELISA kit
Specificity This kit recognizes Human CP in samples. No significant cross-reactivity or interference between Human CP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 95
EDTA plasma (n=8) 90-102 96
Cell culture media (n=8) 85-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (μg/mL) 2.02 5.90 21.85 2.23 5.36 20.31
Standard deviation 0.12 0.29 1.11 0.11 0.26 0.87
CV (%) 5.94 4.92 5.08 4.93 4.85 4.28
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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