产品详情

  • 产品名称：Human CLEC4E (C-type lectin domain family 4 member E) Sandwich ELISA (STJE0002535)

  • 产品型号：STJE0002535
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CLEC4E (C-type lectin domain family 4 member E) Sandwich ELISA (STJE0002535)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This CLEC4E Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CLEC4E
Gene ID: 26253
Uniprot ID: CLC4E_HUMAN
Specificity: This kit recognizes Human CLEC4E in samples. No significant cross-reactivity or interference between Human CLEC4E and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue.
Protein Name C-Type Lectin Domain Family 4 Member E
C-Type Lectin Superfamily Member 9
Macrophage-Inducible C-Type Lectin
Mincle
Database Links Reactome: R-HSA-5621480
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Cell Projection
Phagocytic Cup
Alternative ELISA Names C-Type Lectin Domain Family 4 Member E ELISA kit
C-Type Lectin Superfamily Member 9 ELISA kit
Macrophage-Inducible C-Type Lectin ELISA kit
Mincle ELISA kit
CLEC4E ELISA kit
CLECSF9 ELISA kit
MINCLE ELISA kit
UNQ218 ELISA kit
PRO244 ELISA kit
Specificity This kit recognizes Human CLEC4E in samples. No significant cross-reactivity or interference between Human CLEC4E and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 98
EDTA plasma (n=8) 90-102 96
Cell culture media (n=8) 93-105 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 244.41 742.23 2143.60 233.43 733.88 2131.05
Standard deviation 12.34 30.21 99.25 15.69 33.39 78.64
CV (%) 5.05 4.07 4.63 6.72 4.55 3.69
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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