产品详情

  • 产品名称：Human CD59 (CD59 glycoprotein) CLIA Kit (STJC0000365)

  • 产品型号：STJC0000365
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CD59 (CD59 glycoprotein) CLIA Kit (STJC0000365)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human CD59 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CD59
Gene ID: 966
Uniprot ID: CD59_HUMAN
Specificity: This kit recognizes Human CD59 in samples. No significant cross-reactivity or interference between Human CD59 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Function Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Protein Name Cd59 Glycoprotein
1f5 Antigen
20 Kda Homologous Restriction Factor
Hrf-20
Hrf20
Mac-Inhibitory Protein
Mac-Ip
Mem43 Antigen
Membrane Attack Complex Inhibition Factor
Macif
Membrane Inhibitor Of Reactive Lysis
Mirl
Protectin
Cd Antigen Cd59
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-977606
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Soluble Form Found In A Number Of Tissues
Alternative CLIA Names Cd59 Glycoprotein CLIA kit
1f5 Antigen CLIA kit
20 Kda Homologous Restriction Factor CLIA kit
Hrf-20 CLIA kit
Hrf20 CLIA kit
Mac-Inhibitory Protein CLIA kit
Mac-Ip CLIA kit
Mem43 Antigen CLIA kit
Membrane Attack Complex Inhibition Factor CLIA kit
Macif CLIA kit
Membrane Inhibitor Of Reactive Lysis CLIA kit
Mirl CLIA kit
Protectin CLIA kit
Cd Antigen Cd59 CLIA kit
CD59 CLIA kit
MIC11 CLIA kit
MIN1 CLIA kit
MIN2 CLIA kit
MIN3 CLIA kit
MSK21 CLIA kit
Specificity This kit recognizes Human CD59 in samples. No significant cross-reactivity or interference between Human CD59 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-117 108
EDTA plasma(n=8) 96-111 104
Cell culture media(n=8) 100-117 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.60 120.88 475.96 54.10 109.30 441.40
Standard deviation 5.40 13.20 50.02 5.46 10.83 48.29
CV (%) 9.89 10.92 10.51 10.09 9.91 10.94
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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