产品详情

  • 产品名称：Human CD55 (Complement decay-accelerating factor) CLIA Kit (STJC0000844)

  • 产品型号：STJC0000844
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CD55 (Complement decay-accelerating factor) CLIA Kit (STJC0000844)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human CD55 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CD55
Gene ID: 1604
Uniprot ID: DAF_HUMAN
Specificity: This kit recognizes Human DAF in samples. No significant cross-reactivity or interference between Human DAF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Post Translational Modifications The Ser/Thr-rich domain is heavily O-glycosylated.
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable). (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
Protein Name Complement Decay-Accelerating Factor
Cd Antigen Cd55
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-977606
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Secreted
Isoform 6: Cell Membrane
Isoform 7: Cell Membrane
Alternative CLIA Names Complement Decay-Accelerating Factor CLIA kit
Cd Antigen Cd55 CLIA kit
CD55 CLIA kit
CR CLIA kit
DAF CLIA kit
Specificity This kit recognizes Human DAF in samples. No significant cross-reactivity or interference between Human DAF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma(n=8) 98-116 106
Cell culture media(n=8) 99-112 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 38.61 81.96 341.71 38.51 84.34 329.84
Standard deviation 3.26 8.16 38.13 4.19 8.53 30.18
CV (%) 8.44 9.96 11.16 10.88 10.11 9.15
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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