产品详情

  • 产品名称：Human CASP1 (Caspase-1) CLIA Kit (STJC0000016)

  • 产品型号：STJC0000016
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human CASP1 (Caspase-1) CLIA Kit (STJC0000016)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.75pg/mL
Detection Limit: 6.25~400pg/mL
Short Description: This human CASP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CASP1
Gene ID: 834
Uniprot ID: CASP1_HUMAN
Specificity: This kit recognizes Human CASP1 in samples. No significant cross-reactivity or interference between Human CASP1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Post Translational Modifications The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. Cleavage in the interdomain linker region is required to induce pyroptosis.
Function Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. Isoform Delta: Apoptosis inactive. Isoform Epsilon: Apoptosis inactive.
Protein Name Caspase-1
Casp-1
Interleukin-1 Beta Convertase
Il-1bc
Interleukin-1 Beta-Converting Enzyme
Ice
Il-1 Beta-Converting Enzyme
P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6803207
Reactome: R-HSA-844456
Reactome: R-HSA-844615
Reactome: R-HSA-844623
Reactome: R-HSA-9008059
Reactome: R-HSA-9660826
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Cell Membrane
Alternative CLIA Names Caspase-1 CLIA kit
Casp-1 CLIA kit
Interleukin-1 Beta Convertase CLIA kit
Il-1bc CLIA kit
Interleukin-1 Beta-Converting Enzyme CLIA kit
Ice CLIA kit
Il-1 Beta-Converting Enzyme CLIA kit
P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 CLIA kit
CASP1 CLIA kit
IL1BC CLIA kit
IL1BCE CLIA kit
Specificity This kit recognizes Human CASP1 in samples. No significant cross-reactivity or interference between Human CASP1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 96
EDTA plasma(n=8) 90-104 97
Cell culture media(n=8) 93-106 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 18.81 49.91 189.93 18.27 45.54 182.67
Standard deviation 1.63 5.07 16.77 1.79 5.41 19.55
CV (%) 8.67 10.16 8.83 9.80 11.88 10.70
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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