产品详情
简单介绍:
Human CANX (Calnexin) Sandwich ELISA (STJE0002355)
详情介绍:
Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.38ng/mL |
Detection Limit: | 0.63~40ng/mL |
Short Description: | This CANX Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | CANX |
Gene ID: | 821 |
Uniprot ID: | CALX_HUMAN |
Specificity: | This kit recognizes Human CNX in samples. No significant cross-reactivity or interference between Human CNX and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. |
Protein Name | Calnexin Ip90 Major Histocompatibility Complex Class I Antigen-Binding Protein P88 P90 |
Database Links | Reactome: R-HSA-168316 Reactome: R-HSA-2132295 Reactome: R-HSA-8984722 Reactome: R-HSA-901042 Reactome: R-HSA-9020956 Reactome: R-HSA-9683686 Reactome: R-HSA-9694548 Reactome: R-HSA-983170 |
Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Melanosome Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv The Palmitoylated Form Preferentially Localizes To The Perinuclear Rough Er |
Alternative ELISA Names | Calnexin ELISA kit Ip90 ELISA kit Major Histocompatibility Complex Class I Antigen-Binding Protein P88 ELISA kit P90 ELISA kit CANX ELISA kit |
Specificity | This kit recognizes Human CNX in samples. No significant cross-reactivity or interference between Human CNX and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 90-104 | 98 |
EDTA plasma (n=8) | 92-104 | 98 |
Cell culture media (n=8) | 94-106 | 101 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/ml) | 1.82 | 5.34 | 15.98 | 1.83 | 5.09 | 17.28 |
Standard deviation | 0.12 | 0.30 | 0.56 | 0.12 | 0.21 | 0.79 |
CV (%) | 6.59 | 5.62 | 3.50 | 6.56 | 4.13 | 4.57 |
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