产品详情

  • 产品名称：Human AQP1 (Aquaporin-1) Sandwich ELISA (STJE0002263)

  • 产品型号：STJE0002263
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human AQP1 (Aquaporin-1) Sandwich ELISA (STJE0002263)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 75pg/mL
Detection Limit: 125~8000pg/mL
Short Description: This AQP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: AQP1
Gene ID: 358
Uniprot ID: AQP1_HUMAN
Specificity: This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane.
Protein Name Aquaporin-1
Aqp-1
Aquaporin-Chip
Urine Water Channel
Water Channel Protein For Red Blood Cells And Kidney Proximal Tubule
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative ELISA Names Aquaporin-1 ELISA kit
Aqp-1 ELISA kit
Aquaporin-Chip ELISA kit
Urine Water Channel ELISA kit
Water Channel Protein For Red Blood Cells And Kidney Proximal Tubule ELISA kit
AQP1 ELISA kit
CHIP28 ELISA kit
Specificity This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma (n=8) 87-100 93
Cell culture media (n=8) 92-103 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 399.90 1101.92 3738.97 437.93 1190.85 3925.00
Standard deviation 20.03 59.28 118.15 26.54 49.18 174.27
CV (%) 5.01 5.38 3.16 6.06 4.13 4.44
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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