产品详情

  • 产品名称：Human APOC3 (Apolipoprotein C-III) Sandwich ELISA (STJE0002248)

  • 产品型号：STJE0002248
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human APOC3 (Apolipoprotein C-III) Sandwich ELISA (STJE0002248)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69ng/mL
Detection Limit: 7.81~500ng/mL
Short Description: This APOC3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: APOC3
Gene ID: 345
Uniprot ID: APOC3_HUMAN
Specificity: This kit recognizes Human ApoC3 in samples. No significant cross-reactivity or interference between Human ApoC3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The most abundant glycoforms are characterized by an O-linked disaccharide galactose linked to N-acetylgalactosamine (Gal-GalNAc), further modified with up to 3 sialic acid residues. Less abundant glycoforms are characterized by more complex and fucosylated glycan moieties. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
Function Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion.extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
Protein Name Apolipoprotein C-Iii
Apo-Ciii
Apoc-Iii
Apolipoprotein C3
Database Links Reactome: R-HSA-8963888
Reactome: R-HSA-8963901
Reactome: R-HSA-8964058
Reactome: R-HSA-975634
Cellular Localisation Secreted
Alternative ELISA Names Apolipoprotein C-Iii ELISA kit
Apo-Ciii ELISA kit
Apoc-Iii ELISA kit
Apolipoprotein C3 ELISA kit
APOC3 ELISA kit
Specificity This kit recognizes Human ApoC3 in samples. No significant cross-reactivity or interference between Human ApoC3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 96
EDTA plasma (n=8) 85-98 90
Cell culture media (n=8) 93-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 26.60 65.80 176.80 25.70 62.10 170.40
Standard deviation 1.80 3.60 6.20 1.60 2.70 5.50
CV (%) 6.77 5.47 3.51 6.23 4.35 3.23
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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