产品详情

  • 产品名称：Human C4BPA (C4b-binding protein alpha chain) CLIA Kit (STJC0000237)

  • 产品型号：STJC0000237
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human C4BPA (C4b-binding protein alpha chain) CLIA Kit (STJC0000237)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This human C4BPA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: C4BPA
Gene ID: 722
Uniprot ID: C4BPA_HUMAN
Specificity: This kit recognizes Human C4BP Alpha in samples. No significant cross-reactivity or interference between Human C4BP Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Chylomicrons in the plasma.
Function Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
Protein Name C4b-Binding Protein Alpha Chain
C4bp
Proline-Rich Protein
Prp
Database Links Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative CLIA Names C4b-Binding Protein Alpha Chain CLIA kit
C4bp CLIA kit
Proline-Rich Protein CLIA kit
Prp CLIA kit
C4BPA CLIA kit
C4BP CLIA kit
Specificity This kit recognizes Human C4BPα in samples. No significant cross-reactivity or interference between Human C4BPα and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-111 102
EDTA plasma(n=8) 95-111 102
Cell culture media(n=8) 97-111 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.93 2.32 7.95 0.89 2.23 8.51
Standard deviation 0.09 0.20 0.76 0.09 0.16 0.59
CV (%) 9.68 8.62 9.56 10.11 7.17 6.93
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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