产品详情

  • 产品名称：Human ASM (Acid Sphingomyelinase) Sandwich ELISA Kit Ready-To-Use (STJE0013712)

  • 产品型号：STJE0013712
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ASM (Acid Sphingomyelinase) Sandwich ELISA Kit Ready-To-Use (STJE0013712)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.117ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This ASM Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: SMPD1
Gene ID: 6609
Uniprot ID: ASM_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Proteolytically processed. Mature lysosomal form arises from C-terminal proteolytic processing of pro-sphingomyelin phosphodiesterase. Sphingomyelin phosphodiesterase, processed form: This form is generated following cleavage by CASP7 in the extracellular milieu. It shows increased activity. Both lysosomal and secreted forms are glycosylated but they show a differential pattern of glycosylation. Phosphorylated at Ser-510 by PRKCD upon stress stimuli. Phosphorylation is required for secretion.
Function Converts sphingomyelin to ceramide. Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly. However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form. In the lysosomes, converts sphingomyelin to ceramide. Plays an important role in the export of cholesterol from the intraendolysosomal membranes. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Modulates stress-induced apoptosis through the production of ceramide. When secreted, modulates cell signaling with its ability to reorganize the plasma membrane by converting sphingomyelin to ceramide. Secreted form is increased in response to stress and inflammatory mediators such as IL1B, IFNG or TNF as well as upon infection with bacteria and viruses. Produces the release of ceramide in the outer leaflet of the plasma membrane playing a central role in host defense. Ceramide reorganizes these rafts into larger signaling platforms that are required to internalize P. aeruginosa, induce apoptosis and regulate the cytokine response in infected cells. In wounded cells, the lysosomal form is released extracellularly in the presence of Ca(2+) and promotes endocytosis and plasma membrane repair. Sphingomyelin phosphodiesterase, processed form: This form is generated following cleavage by CASP7 in the extracellular milieu in response to bacterial infection. It shows increased ability to convert sphingomyelin to ceramide and promotes plasma membrane repair. Plasma membrane repair by ceramide counteracts the action of gasdermin-D (GSDMD) perforin (PRF1) pores that are formed in response to bacterial infection. (Microbial infection) Secretion is activated by bacteria such as P. aeruginos, N. gonorrhoeae and others, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection. (Microbial infection) Secretion is activated by human coronaviruses SARS-CoV and SARS-CoV-2 as well as Zaire ebolavirus, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection. Isoform 2: Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity. Isoform 3: Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.
Protein Name Sphingomyelin Phosphodiesterase
Acid Sphingomyelinase
Asmase Cleaved Into - Sphingomyelin Phosphodiesterase - Processed Form
Database Links Reactome: R-HSA-1660662
Cellular Localisation Lysosome
Lipid Droplet
Secreted
The Secreted Form Is Induced In A Time- And Dose-Dependent By Il1b And Tnf As Well As Stress And Viral Infection
This Increase Of The Secreted Form Seems To Be Due To Exocytosis Of The Lysosomal Form And Is Ca(2+)-Dependent
Secretion Is Dependent Of Phosphorylation At Ser-510
Secretion Is Induced By Inflammatory Mediators Such As Il1b
Ifng Or Tnf As Well As Infection With Bacteria And Viruses
Sphingomyelin Phosphodiesterase
Processed Form: Secreted
Extracellular Space
This Form Is Generated Following Cleavage By Casp7
Alternative ELISA Names Sphingomyelin Phosphodiesterase ELISA kit
Acid Sphingomyelinase ELISA kit
Asmase Cleaved Into - Sphingomyelin Phosphodiesterase - Processed Form ELISA kit
SMPD1 ELISA kit
ASM ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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