产品详情

  • 产品名称：Human ARHGDIA (Rho GDP-dissociation inhibitor 1) CLIA Kit (STJC0000289)

  • 产品型号：STJC0000289
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ARHGDIA (Rho GDP-dissociation inhibitor 1) CLIA Kit (STJC0000289)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human ARHGDIA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: ARHGDIA
Gene ID: 396
Uniprot ID: GDIR1_HUMAN
Specificity: This kit recognizes Human ARHGDI Alpha in samples. No significant cross-reactivity or interference between Human ARHGDI Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Protein Name Rho Gdp-Dissociation Inhibitor 1
Rho Gdi 1
Rho-Gdi Alpha
Database Links Reactome: R-HSA-193634
Reactome: R-HSA-209563
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Cellular Localisation Cytoplasm
Alternative CLIA Names Rho Gdp-Dissociation Inhibitor 1 CLIA kit
Rho Gdi 1 CLIA kit
Rho-Gdi Alpha CLIA kit
ARHGDIA CLIA kit
GDIA1 CLIA kit
Specificity This kit recognizes Human ARHGDIα in samples. No significant cross-reactivity or interference between Human ARHGDIα and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-116 108
EDTA plasma(n=8) 97-113 104
Cell culture media(n=8) 86-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 105.47 293.06 765.78 108.88 279.17 800.98
Standard deviation 8.87 26.05 64.78 13.41 31.74 80.74
CV (%) 8.41 8.89 8.46 12.32 11.37 10.08
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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