产品详情

  • 产品名称：Human ALB (Albumin) CLIA Kit (STJC0000910)

  • 产品型号：STJC0000910
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ALB (Albumin) CLIA Kit (STJC0000910)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5ng/mL
Detection Limit: 62.5~4000ng/mL
Short Description: This human ALB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: ALB
Gene ID: 213
Uniprot ID: ALBU_HUMAN
Specificity: This kit recognizes Human ALB in samples. No significant cross-reactivity or interference between Human ALB and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Plasma.
Post Translational Modifications Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606. Glycated in diabetic patients. Phosphorylated by FAM20C in the extracellular medium. Acetylated on Lys-223 by acetylsalicylic acid.
Function Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
Protein Name Albumin
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-159418
Reactome: R-HSA-189451
Reactome: R-HSA-189483
Reactome: R-HSA-2168880
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Reactome: R-HSA-8964058
Reactome: R-HSA-9707564
Reactome: R-HSA-9749641
Reactome: R-HSA-9757110
Reactome: R-HSA-9793528
Cellular Localisation Secreted
Alternative CLIA Names Albumin CLIA kit
ALB CLIA kit
GIG20 CLIA kit
GIG42 CLIA kit
PRO0903 CLIA kit
PRO1708 CLIA kit
PRO2044 CLIA kit
PRO2619 CLIA kit
PRO2675 CLIA kit
UNQ696 CLIA kit
PRO1341 CLIA kit
Specificity This kit recognizes Human ALB in samples. No significant cross-reactivity or interference between Human ALB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 96
EDTA plasma(n=8) 100-111 105
Cell culture media(n=8) 98-115 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 189.31 444.15 1789.00 171.78 400.53 1841.83
Standard deviation 16.45 35.22 192.14 20.48 32.88 130.77
CV (%) 8.69 7.93 10.74 11.92 8.21 7.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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