产品详情

  • 产品名称：Human AFP (Alpha-fetoprotein) Sandwich ELISA (STJE0006756)

  • 产品型号：STJE0006756
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human AFP (Alpha-fetoprotein) Sandwich ELISA (STJE0006756)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78-50ng/mL
Short Description: This AFP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual.
Assay Time: 1.5h
Detection: Colormetric
Gene Symbol: AFP
Gene ID: 174
Uniprot ID: FETA_HUMAN
Specificity: This kit recognizes Human AFP in samples. No significant cross-reactivity or interference between Human AFP and analogues was observed.
Sample Type: Serum and plasma
Post Translational Modifications Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated.
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Protein Name Alpha-Fetoprotein
Alpha-1-Fetoprotein
Alpha-Fetoglobulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative ELISA Names Alpha-Fetoprotein ELISA kit
Alpha-1-Fetoprotein ELISA kit
Alpha-Fetoglobulin ELISA kit
AFP ELISA kit
HPAFP ELISA kit
Specificity This kit recognizes αFP in samples. No significant cross-reactivity or interference between αFP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T 2-8℃, 1 months
Reference Standard 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×) 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL 2-8℃ (Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×) 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃ (Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 99
EDTA plasma(n=8) 87-98 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.40 6.58 17.73 2.29 6.46 16.31
Standard deviation 0.13 0.32 0.97 0.14 0.32 0.74
CV (%) 5.23 4.90 5.45 6.22 5.00 4.53
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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