产品详情

  • 产品名称：Human ADP (Adiponectin) Sandwich ELISA Kit (STJE0006951)

  • 产品型号：STJE0006951
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ADP (Adiponectin) Sandwich ELISA Kit (STJE0006951)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.65ng/mL
Detection Limit: 1.56-100ng/mL
Short Description: This ADP Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: ADIPOQ
Gene ID: 9370
Uniprot ID: ADIPO_HUMAN
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Post Translational Modifications HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. Succination of Cys-36 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mammals. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes.
Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Protein Name Adiponectin
30 Kda Adipocyte Complement-Related Protein
Adipocyte Complement-Related 30 Kda Protein
Acrp30
Adipocyte - C1q And Collagen Domain-Containing Protein
Adipose Most Abundant Gene Transcript 1 Protein
Apm-1
Gelatin-Binding Protein
Database Links Reactome: R-HSA-163680
Reactome: R-HSA-381340
Cellular Localisation Secreted
Alternative ELISA Names Adiponectin ELISA kit
30 Kda Adipocyte Complement-Related Protein ELISA kit
Adipocyte Complement-Related 30 Kda Protein ELISA kit
Acrp30 ELISA kit
Adipocyte - C1q And Collagen Domain-Containing Protein ELISA kit
Adipose Most Abundant Gene Transcript 1 Protein ELISA kit
Apm-1 ELISA kit
Gelatin-Binding Protein ELISA kit
ADIPOQ ELISA kit
ACDC ELISA kit
ACRP30 ELISA kit
APM1 ELISA kit
GBP28 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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