产品详情

  • 产品名称：Human ADA (Adenosine deaminase) Sandwich ELISA (STJE0002084)

  • 产品型号：STJE0002084
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ADA (Adenosine deaminase) Sandwich ELISA (STJE0002084)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This ADA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ADA
Gene ID: 100
Uniprot ID: ADA_HUMAN
Specificity: This kit recognizes Human ADA in samples. No significant cross-reactivity or interference between Human ADA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development.
Protein Name Adenosine Deaminase
Adenosine Aminohydrolase
Database Links Reactome: R-HSA-74217
Reactome: R-HSA-9734735
Reactome: R-HSA-9755088
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Cell Junction
Cytoplasmic Vesicle Lumen
Cytoplasm
Lysosome
Colocalized With Dpp4 At The Cell Surface
Alternative ELISA Names Adenosine Deaminase ELISA kit
Adenosine Aminohydrolase ELISA kit
ADA ELISA kit
ADA1 ELISA kit
Specificity This kit recognizes Human ADA in samples. No significant cross-reactivity or interference between Human ADA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 97
EDTA plasma (n=8) 88-103 95
Cell culture media (n=8) 87-100 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.07 2.61 9.80 1.10 2.65 10.78
Standard deviation 0.07 0.11 0.30 0.07 0.11 0.50
CV (%) 6.54 4.21 3.06 6.36 4.15 4.64
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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